Journal: PLoS ONE

Article Title: Regulation of protein-coding gene and long noncoding RNA pairs in liver of conventional and germ-free mice following oral PBDE exposure

doi: 10.1371/journal.pone.0201387

Figure Lengend Snippet: A. Top: a pie chart showing lncRNAs that were not expressed in any groups (blue), stably expressed in all groups (grey), and differentially expressed by PBDE treatment in at least one of the four comparisons (orange). These four comparisons are: CV_CO (corn oil) vs CV_BDE-47; CV_CO vs CV_BDE-99; GF_CO vs GF_BDE-47; and GF_CO vs GF_BDE-99, FDR adjusted p-value <0.05). Down: a Venn diagram showing the differentially expressed lncRNAs that were commonly or uniquely regulated by PBDEs among the four comparisons as described in . Venn diagram was generated using JMP Genomics. B-C. lncRNAs that were differentially regulated by both PBDEs in livers of CV and GF mice. B. lncRNAs that were commonly up-regulated or down-regulated by BDE-47 and BDE-99 in livers of both CV and GF mice. C. lncRNAs that were consistently down-regulated by BDE-47 in livers of CV and GF mice, but were oppositely regulated in CV (down-regulation) and GF (up-regulation) mice by BDE-99. Asterisks (*) indicate statistically significant differences as compared to vehicle-treated groups of the same enterotypes of mice (Cuffdiff, p < 0.05).

Article Snippet: The Venn diagrams of the differentially expressed PCGs and lncRNAs were generated based on their standardized mean FPKM values, using the JMP Genome Software (SAS Institute, Cary, NC).

Techniques: Stable Transfection, Generated